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ERX1845369: Illumina HiSeq 2500 sequencing; qiita_ptid_2365:10793.10533.Rac.23.2a
1 ILLUMINA (Illumina HiSeq 2500) run: 199,368 spots, 24.6M bases, 11.5Mb downloads

Design: Sponge Microbiome Project
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: The Sponge Microbiome Project
show Abstracthide Abstract
Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide a dataset that will facilitate microbiome comparisons across large spatial, temporal and environmental scales.
Sample: 10793.10533.Rac.23.2a; SpongeMicrobiome Project
SAMEA46753168 • ERS1500452 • All experiments • All runs
Library:
Name: 10793.10533.Rac.23.2a
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: EMP protocol, V4 515fbc/806r HiSeq
Experiment attributes: (show all 12 attributes...) (hide...)
barcode: CGTAGAGCTCTC
center_name: EMP sponges round 1
lane_round: Lane 5 2nd round
linker: GT
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer: GTGTGCCAGCMGCCGCGGTAA
run_center: CCME
run_date: 11/18/14
run_prefix: lane5_Undetermined_L005
sequencing_meth: sequencing by synthesis
target_gene: 16S rRNA
target_subfragment: V4
Runs: 1 run, 199,368 spots, 24.6M bases, 11.5Mb
Run# of Spots# of BasesSizePublished
ERR1780815199,36824.6M11.5Mb2017-06-21

ID:
4201372

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